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A simple in vivo model of collagen degradation using collagen-gelled cotton buds: The effects of collagenase inhibitors and other agents

Identifieur interne : 002B90 ( Main/Exploration ); précédent : 002B89; suivant : 002B91

A simple in vivo model of collagen degradation using collagen-gelled cotton buds: The effects of collagenase inhibitors and other agents

Auteurs : Eric H. Karran [Royaume-Uni] ; Kathryn Dodgson [Royaume-Uni] ; Sonia J. Harris [Royaume-Uni] ; Roger E. Markwell [Royaume-Uni] ; Gregory P. Harper [Royaume-Uni]

Source :

Inflammation Research [ 1023-3830 ] ; 1995-01-01.

RBID : ISTEX:FE4CEFDB574A3470D0D3CFD21E170613DBC45D41

English descriptors

Abstract

Abstract: A simplein vivo model of collagen degradation has been developed, and the effects of various agents have been tested. Type I collagen was prepared from rat skin and acetylated with either [3H]- or [14C] acetic anhydride. The radiolabelled collagen was added to sterile cotton buds and incubated at 37°C to allow the collagen to form native fibrils that were firmly adsorbed to the cotton matrix. After subcutaneous implantation of the collagengelled cotton buds into rats, the radiolabelled collagen was progressively removed over a period of weeks by an infiltrating granuloma. Of the agents that were administered directly into the cotton buds using subcutaneously implanted osmotic mini-pumps, only the synthetic collagenase inhibitors CI-A (containing a hydroxamate moiety as a zinc ligand) and CI-C (containing a thiol moiety as a zinc ligand) were able to prevent the removal of collagen: their efficacy correlated with the level of collagenase inhibitory activity assayed in the exudate fluid sequestered within the cotton bud granuloma. Of the agents that were administered systemically, including anti-inflammatory drugs and other compounds used as therapies for arthritis, only hydrocortisone was able to inhibit the removal of radiolabelled collagen. These results suggest that, in this model, interstitial collagenase, a member of the matrix metalloproteinase family, comprised the major degradative pathway for collagen. The collagen-gelled cotton bud model is a useful test system for delineating those processes that result in collagen catabolism. In addition, the model can be used for testing agents, including those of limited or unknown systemic bioavailability, in order to discover novel therapeutic agents for preventing collagen degradation in connective tissue diseases such as arthritis.

Url:

https://api.istex.fr/ark:/67375/1BB-90162KKV-K/fulltext.pdf

DOI: 10.1007/BF01630486

Affiliations:

Royaume-Uni

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Le document en format XML

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<author><name sortKey="Dodgson, Kathryn" sort="Dodgson, Kathryn" uniqKey="Dodgson K" first="Kathryn" last="Dodgson">Kathryn Dodgson</name>
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<author><name sortKey="Harris, Sonia J" sort="Harris, Sonia J" uniqKey="Harris S" first="Sonia J." last="Harris">Sonia J. Harris</name>
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<series><title level="j">Inflammation Research</title>
<title level="j" type="abbrev">Inflamm Res</title>
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<term>Collagen</term>
<term>Collagenase inhibitors</term>
<term>Metalloproteinase</term>
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<keywords scheme="Teeft" xml:lang="en"><term>Aliquot</term>
<term>Alpha macroglobulin</term>
<term>Arthritic disease</term>
<term>Arthritis</term>
<term>Arthritis rheum</term>
<term>Articular</term>
<term>Articular cartilage</term>
<term>Assay</term>
<term>Biol chem</term>
<term>Bud</term>
<term>Calcium chloride</term>
<term>Cartilage</term>
<term>Cartilage degradation</term>
<term>Collagen</term>
<term>Collagen catabolism</term>
<term>Collagen degradation</term>
<term>Collagen network</term>
<term>Collagen removal</term>
<term>Collagenase</term>
<term>Collagenase activity</term>
<term>Collagenase inhibitors</term>
<term>Collagengelled cotton buds</term>
<term>Contralateral control</term>
<term>Control group</term>
<term>Cotton buds</term>
<term>Cotton matrix</term>
<term>Degradation</term>
<term>Endogenous</term>
<term>Endogenous inhibitor levels</term>
<term>Exudate</term>
<term>Exudate fluid</term>
<term>Exudate fluids</term>
<term>Female rats</term>
<term>Fibril</term>
<term>Form fibrils</term>
<term>Granuloma</term>
<term>Granulomatous</term>
<term>Granulomatous tissue</term>
<term>Human collagenase</term>
<term>Hydrocortisone</term>
<term>Implantation</term>
<term>Infusion</term>
<term>Infusion period</term>
<term>Infusion rate</term>
<term>Inhibitor</term>
<term>Karran</term>
<term>Liquid scintillation</term>
<term>Macroglobulin</term>
<term>Matrix</term>
<term>Matrix degradation</term>
<term>Matrix metalloproteinase inhibitors</term>
<term>Matrix metalloproteinases</term>
<term>Metalloproteinase</term>
<term>Metalloproteinases</term>
<term>Methylamine</term>
<term>Microfuge tubes</term>
<term>Microtitre plates</term>
<term>Native collagen</term>
<term>Other agents</term>
<term>Papain</term>
<term>Proteinase</term>
<term>Proteoglycan</term>
<term>Radiolabelled</term>
<term>Radiolabelled collagen</term>
<term>Rheum</term>
<term>Rheumatoid</term>
<term>Rheumatoid arthritis</term>
<term>Right flank</term>
<term>Scintillation</term>
<term>Smithkline beecham</term>
<term>Subcutaneously</term>
<term>Synthetic collagenase inhibitors</term>
<term>Trypsin</term>
<term>Vehicle control group</term>
<term>Vivo</term>
<term>Vivo model</term>
<term>Water bath</term>
</keywords>
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<langUsage><language ident="en">en</language>
</langUsage>
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<front><div type="abstract" xml:lang="en">Abstract: A simplein vivo model of collagen degradation has been developed, and the effects of various agents have been tested. Type I collagen was prepared from rat skin and acetylated with either [3H]- or [14C] acetic anhydride. The radiolabelled collagen was added to sterile cotton buds and incubated at 37°C to allow the collagen to form native fibrils that were firmly adsorbed to the cotton matrix. After subcutaneous implantation of the collagengelled cotton buds into rats, the radiolabelled collagen was progressively removed over a period of weeks by an infiltrating granuloma. Of the agents that were administered directly into the cotton buds using subcutaneously implanted osmotic mini-pumps, only the synthetic collagenase inhibitors CI-A (containing a hydroxamate moiety as a zinc ligand) and CI-C (containing a thiol moiety as a zinc ligand) were able to prevent the removal of collagen: their efficacy correlated with the level of collagenase inhibitory activity assayed in the exudate fluid sequestered within the cotton bud granuloma. Of the agents that were administered systemically, including anti-inflammatory drugs and other compounds used as therapies for arthritis, only hydrocortisone was able to inhibit the removal of radiolabelled collagen. These results suggest that, in this model, interstitial collagenase, a member of the matrix metalloproteinase family, comprised the major degradative pathway for collagen. The collagen-gelled cotton bud model is a useful test system for delineating those processes that result in collagen catabolism. In addition, the model can be used for testing agents, including those of limited or unknown systemic bioavailability, in order to discover novel therapeutic agents for preventing collagen degradation in connective tissue diseases such as arthritis.</div>
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A simple in vivo model of collagen degradation using collagen-gelled cotton buds: The effects of collagenase inhibitors and other agents

A simple in vivo model of collagen degradation using collagen-gelled cotton buds: The effects of collagenase inhibitors and other agents

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri